Fig. 1

Concurrent PAUS microscopy. a The schematic of the PAUS microscopy system. LR, laser; L, lens; M, mirror; UST/R, ultrasonic pulser-receiver; Amp, amplifier; WT, water tank with transparent bottom. The mouse was placed under a small water tank on a 3D motorized translation stage (not shown) with the hind leg fixed on a sample holder. b Experimental timing sequence chart. A LabVIEW program sends out triggers to the laser, ultrasonic pulser-receiver, stage, and DAQ. c, d Structure and photo of the DE-UST with a central aperture. The high frequency element (40 MHz) is inside and the low frequency element (20 MHz) is outside. e Detailed timing sequence. Trigger 1 is for 532 nm laser firing, followed by a DAQ trigger that starts data acquisition for the PA signal. Trigger 2 is for US transmission, followed by a DAQ trigger that starts data acquisition for the US signal. The A-line acquisition frequency is 1000 Hz, and the sampling frequency for each A-line is 250 MHz