 Review
 Open Access
 Published:
Recent methodology advances in fluorescence molecular tomography
Visual Computing for Industry, Biomedicine, and Art volume 1, Article number: 1 (2018)
Abstract
Molecular imaging (MI) is a novel imaging discipline that has been continuously developed in recent years. It combines biochemistry, multimodal imaging, biomathematics, bioinformatics, cell & molecular physiology, biophysics, and pharmacology, and it provides a new technology platform for the early diagnosis and quantitative analysis of diseases, treatment monitoring and evaluation, and the development of comprehensive physiology. Fluorescence Molecular Tomography (FMT) is a type of optical imaging modality in MI that captures the threedimensional distribution of fluorescence within a biological tissue generated by a specific molecule of fluorescent material within a biological tissue. Compared with other optical molecular imaging methods, FMT has the characteristics of high sensitivity, low cost, and safety and reliability. It has become the research frontier and research hotspot of optical molecular imaging technology. This paper took an overview of the recent methodology advances in FMT, mainly focused on the photon propagation model of FMT based on the radiative transfer equation (RTE), and the reconstruction problem solution consist of forward problem and inverse problem. We introduce the detailed technologies utilized in reconstruction of FMT. Finally, the challenges in FMT were discussed. This survey aims at summarizing current research hotspots in methodology of FMT, from which future research may benefit.
Background
Optical molecular imaging (OMI) uses molecular probes to label target organisms. Under certain external conditions, the molecular probe releases fluorescent light in the visible or nearinfrared spectrum, using highsensitivity detection equipment for fluorescence. The signal is collected and the position and intensity of the fluorescent light source are displayed to obtain physiological activity information of the organism’s molecular cells. OMI has the advantages of high sensitivity, no radiation, low cost, dynamic observation, and intuitive imaging, and can achieve early detection of tumors. Therefore, OMI has been rapidly developed in recent years, and has been widely used in tumor detection, drug development, imageguide surgery and other fields [1,2,3,4].
Fluorescence molecular tomography (FMT) is a threedimensional imaging method based on fluorescence molecular imaging (FMI), which is based on the distribution of fluorescence in biological tissues [2, 3, 5,6,7,8,9,10]. It develops from twodimensional (2D) qualitative imaging to threedimensional (3D) quantitative research, and further expands the integration of stimulated fluorescence in the diagnosis and treatment of cancer, preclinical and clinical applications such as pharmacokinetics. Compared to other OMI technologies, FMT has the characteristics of low cost, safety, reliability, high signal strength, and flexible and reliable imaging. It has developed rapidly in recent years and has become a research frontier and research hotspot for OMI technology [11,12,13,14,15,16,17].
Methods
In FMT, first we need to get the precise photon propagation model for describing both excitation and emission light transmission invivo. When the imaging spatial data needed for FMT reconstruction is obtained, and the reconstruction of the structural data and optical data based on the biological model can be carried out [18]. In general, the image reconstruction process includes two steps: solving the forward problem and solving the inverse problem. The solution of the forward problem is to calculate the photon propagation model of the fluorescence transmitted in the imaging space to obtain the linear relationship between the fluorescence measurement data on the surface of the tissue and the fluorescence distribution inside the bio tissue. After the linear relationship is obtained by solving the photon transfer model, various methods are used to solve the linear model, and the distribution of fluorescence inside the imaging space is obtained, which is called inverse problem [6]. As a conclusion, there are mainly three components in FMT methodology, photon propagation model, forward problems solving and inverse problems solving. Next, we will briefly introduce the mainstream of current methods used in FMT reconstruction, and discusses the analysis of the characteristics of each method and its scope.
Results and discussion
Photon propagation model
The process of transmitting fluorescence from a light source to a biological body through a specific biological tissue is extremely complicated, and includes various physical processes such as scattering of light, intertissue reflection, refraction, diffusion and absorption. For FMT imaging, imaging is usually performed in the visible and nearinfrared optical bands, and the scattering and absorption effects of this band of light inside the biological tissues are the main forms of our study. Therefore, the FMT photon propagation model can be simplified to a photon stochastic propagation model that contains only the scattering and absorption effects without considering the reflection and refraction of different tissues. Today’s mainstream mathematical theories to solve these problems include analytical theory and transport theory. The analytical theory is based on the Maxwell wave equation and takes into account the multiplicative scattering, wave interference and diffraction, and tissue absorption effects of photons, introduces the related physical processes into the wave equation, and through rigorous analytical derivation, the micro integral equation. However, in the actual FMT experiment, the calculus equations containing these complex effects are difficult to solve due to the large number of optical parameters to be obtained, and it is impossible to get exact solutions of these optical parameters at the same time. It can be utilized only in the combination of some a priori knowledge of the circumstances, while narrow the scope of the solution of these optical parameters. Transport theory is based on Boltzmann’s Radiative Transfer Equation (RTE) [19], which is equivalent to photon propagation as transport of photon flux in a medium, from particle fluctuation to energy transport, to study transport of light energy in biological tissues problem. The theory pioneered by Arthur Schuster in 1903, first applied to the theory of gas dynamics and neutron transport. This method is mathematically lax due to ignoring the complex wave effects of photon transmission. However, due to the greatly simplified equations, the theory can flexibly handle the energy transfer phenomena in many random media [20] and is now widely used in many research fields [21,22,23].
The above formula is the RTE timedomain expression. Where r denotes a certain point in the imaging space, Ω denotes the imaging space, c denotes the photon propagation rate in the biological tissue, 푡 denotes the time parameter, \( u\left(r,\widehat{s},t\right) \)denotes Radiance whose dimension is Wm^{−2}sr^{− 1}Hz^{− 1}; μ_{a} and μ_{s} denote the absorption and scattering coefficients respectively representing the transmission of photons in the biological tissue. \( p\left({\widehat{s}}^{\prime },\widehat{s}\right) \) denotes the normalized phase function representing no photonic phase, indicating that a single photon in a single scattering from the probability of scattering in the direction apparently satisfies the probability integral in the spatial domain of 1, ie. \( {\int}_{4\pi }p\left({\widehat{s}}^{\prime },\widehat{s}\right)\mathrm{d}{\widehat{s}}^{\prime }=1 \). \( q\left(r,\widehat{s},t\right) \) denotes the spatial and angular distribution of the fluorescence to be sought [24,25,26].
The above radiation transfer equation is based on conservation of energy and is a very complex calculus equation. In threedimensional biological tissue, the solution of RTE is transformed into a sixdimensional spacetime problem. There are few methods in solving mathematical and computer problems, and it is usually not able to directly close the analytical solution. Moreover, because of its unknowns, it can be solved precisely only in rare cases. Usually it can not get a closed analytical solution. At the same time, it is extremely difficult to solve RTE directly, while the exact solution will only exist in rare cases. Therefore, it is common practice to replace itself with a simplified approximation of the radiation transfer equation [27].
Diffusion Equation (DE) is a widely used RTEbased simplified model [28,29,30,31,32,33,34]. It uses the firstorder spherical harmonic function to expand the important function items in the RTE equation and performs the approximate processing, which significantly reduces the computational complexity and is suitable for the visible and nearinfrared bands of the FMT imaging. The researches show that in the visible and nearinfrared light bands, the result of DE and RTE has high similarity, so it becomes the mainstream model of optical imaging. In principle, DE is based on the approximation of RTE where the photon scattering coefficient is much greater than the absorption coefficient (μ_{a}<<μ_{s}΄). When photons do not meet these characteristics, such as the cavity inside a living organism, or highly scattering tissue like liver, it is difficult to obtain the exact solution of the DE equation. In addition, the diffusion equation takes the nearsource error in describing the photon propagation near the light source region [7], which limits the application range of the diffusion equation.
Based on the above drawbacks of DE equations, in recent years, researchers have proposed such highorder approximations as RTE [7, 35,36,37,38,39,40,41]. Compared with diffusion equations, higherorder approximation models can significantly improve FMT accuracy. The SN model [42], the PN model [43], and the SPN model [41] are three commonly used RTE highorder approximation models and usually give more accurate RTE solutions to the more diffusive equations. By these approximation methods, the traditional RTE equation can be transformed into several coupled higherorder partial differential equations for easy calculation and solution. For example, two coupled equations of N (N + 2) and (N + 1) can be obtained using the SN model and the PN model, respectively. Here, N represents the number of Legendre Polynomials and direction cosines used for SN and PN approximation. As N increases, the approximate order increases. In recent years, SN models and PN models applied to optical molecular imaging have achieved good results [35, 44]. However, the RTE approximate solution based on the SN model and the PN model needs to solve multiple higher order partial differential equations at the same time, which causes great computational cost and limits its practical application. The SPN model is considered to be a good solution to the approximation of diffusion equations and to obtain the improved RTE solution. The SPN model only needs to solve (N + 1)/2 equations, much smaller than the SN model and the PN model, which improves its practical application. Taking N = 7 as an example, we only need to solve four coupled partial differential equations, the coupling equation is as follows [38]:
In addition, in order to overcome the defect of the diffusion equation, the researchers also proposed some mixed photon propagation models that incorporate diffusion equations and other models. For example, The Radiositydiffusion Model can be used to describe photon propagation in nonscattering regions [45]. However, the model is ineffective in lowscattering regions. In addition, Monte Carlo (MC) and the diffusion equation model (MCDE) are also reported [46,47,48]. In the MCDE model, the photon propagation near the light source is obtained by MC simulation, while in other imaging regions diffusion equations are used to describe this. Although MC describes photon transmission more accurately, its computational load is larger, which seriously affects the computational efficiency of MCDE [49]. Accordingly, T. Tarvainen et al. proposed a mixed model combining RTE and DE equations [49]. The hybrid model uses RTE to describe photon transmission in the imaging region that does not satisfy the DE assumption, and the remaining regions are described using DE. The model can effectively characterize the photon propagation properties in highscattering regions and correct the defects such as nearsource error of DE. The results show that the hybrid model can approximate the same accuracy as RTE, and the computational efficiency can be effectively improved compared with RTE.
These reported photon propagation models have been widely used in FMT and achieved good results. However, computational efficiency and accuracy of photon propagation models require further study to significantly improve the accuracy and efficiency of FMT reconstruction. In addition, different photon propagation models need to be developed for different wavelengths and different imaging area sizes.
Forward problem solving
The linear relationship between the measured data on the surface of the imaging area and the internal fluorescence distribution in the imaging area based on the photon propagation model is the core of the FMT forward problem. In recent years, researchers have proposed various mathematical solution methods including analytic method, statistical method and numerical analysis method to solve the forward problem of FMT [6, 7]. The analytical solution to RTE and its many approximation models is usually based on the Green’s function. The Green’s equation sets the light source to a δequation, and on the basis of which the light source is convolution expanded until it fills the entire imaging space [43]. Parsing solves quickly, but is limited to some special cases, such as homogeneous model contains simple and regular objects. Although analytic methods have been extended to imaging spaces of more complex geometries, such as multilayered homogeneous plates [50], this method needs further study in FMT in complicated threedimensional imaging space, especially in the imaging space containing complex geometry happening. Recently, the Kirchhoff Approximation (KA) has been utilized to solve the forward problem of FMT [51, 52]. Compared with the traditional analytical method, the KA has achieved relatively high computation efficiency, and high adaption to complex geometric models.
MC is a classical statistical method for solving photon propagation, which obtains photon propagation properties in imaging space by tracking the propagation of a large number of independent photons. Therefore, this method can be applied directly to solving RTE and is considered as the gold standard for solving forward problems [53]. MC is easy to implement and does not require excessive computational constraints, however, it requires relatively repetitive calculations, and reliable statistical results can only be obtained by computing large sample quantities of photons. Computing speed and memory footprint are the main factors that limit MC applications. In recent years, researchers have proposed a MC method based on GPU (Graphic Processing Unit) hardware acceleration [54,55,56], which significantly improves the computational speed compared with the singleCPU MC method. Meanwhile, the GPUbased MC simulation reconstruction algorithm has also been successfully applied to FMT reconstruction [55, 57].
Compared with analytic method and statistical method, numerical analysis method is the main solving method currently used in optical molecular imaging reconstruction. Its computational efficiency is high and its applicability is wide. Numerical analysis methods include Finite Difference Method (FDM) [40], Boundary Element Method (BEM), Finite Element Method (FEM) [37, 58] and Meshless Method (MM) method [59]. FDM uses equidistant grid points and regular grids to solve the forward problem, which is more efficient than irregular grids. However, FDM has difficulty in dealing with geometrically complex imaging spaces and boundary conditions. In contrast, FEM is the mainstream solution to FMT forward problems in recent years. The main advantage of FEM is its effectiveness in dealing with complex geometric problems. In addition, the system matrices obtained by FEM are usually sparse and positive definite, which leads to a more stable solution and high computational efficiency, which is also beneficial to FMT reconstruction [60, 61]. However, the main drawback of FEM is that it is difficult to generate FEM grid. In contrast, BEM only needs to discretize the imaging surface and the boundaries of the heterogeneous tissue within the space without the need to mesh the entire imaging space.
Therefore, compared with FEM, BEM can effectively reduce the computational dimension and complexity, to improve computational efficiency. However, fast and stable 3D mesh generation for complex geometry problems remains a challenging issue. In order to overcome the problem of 3D mesh generation, Y. An et al. proposed a meshless method [62] and applied it to solve the forward problem of FMT, and the result is shown in Fig. 1. The method only needs to obtain nodes that are relatively independent from each other to discretize the imaging space and does not require a cumbersome gridding process.
Regardless of which method is used to solve the photon propagation model, a linear relationship describing the threedimensional fluorescence distribution of the surface measurement data within the imaging space during FMT imaging is finally obtained as shown in the following equation:
Among them, A ∈ R^{m × n} denotes the FMT system matrix, depicting the photon in the imaging space within the discrete nodes on the transmission characteristics. m is the number of surface measurement data, n is the number of internal nodes in the imaging space. X ∈ R^{n} represents the desired target value of each discrete node inside the imaging space. b ∈ R^{m} denotes the surface measurement data vector. Therefore, solving Eq. (6) is the core issue of FMT reconstruction, that is, the core problem of FMT inverse problem solving.
Inverse problem solving
In FMT preclinical and clinical trials, b in eq. (6) is usually only measured from the imaging surface. However, the dimension of the measurement data on the imaging space surface is usually much less than the number of internal nodes in the imaging space, ie m>>n. Therefore, solving Eq. (6) is illconditioned [33]. Although the excitation fluorescence can be combined by multiple excitationemission equations, the illcondition of the inverse problem can be alleviated to a certain extent [63, 64]. However, the effective information contained in each excitationemission system usually repeats each other and cannot be used as an effective solution information. Therefore, it cannot eliminate the illcondition. Moreover, because of the high scattering properties of photons in the imaging space, Eq. 6 is illposedness and it is difficult to find the exact solution [65,66,67]. At the same time, the measurement noise generated during the experiment also affects the accuracy of the FMT reconstruction [68].
The illposedness of the FMT inverse problem is mainly due to the lack of information and uncertainty due to the high scattering of photons. In order to overcome the illposedness of reverse problems, researchers started from the light source prior information and combined with a variety of a priori information related to the light source and photon transmission to reduce the uncertainty of the information so as to improve the accuracy of inverse problem solving [15, 30, 69,70,71,72,73,74,75,76,77,78]. Feasible region is one of the earlier applied to the optical reconstruction of the information [79, 80]. The main idea is to infer the light source in the imaging area by the location of the light spot area generated by the surface measurement to approximate the area, so as to remove the nodes in other nonapproximate regions in the imaging space, reduce the number of unknowns in Eq. (6) to handle the illposedness. However, the FMT imaging process is different from other imaging modalities due to the influence of the excitation light position. Usually, the spot generated by the surface of the living body has a strong correlation with the position of the excitation light. Therefore, it is determined directly from the surface spot that the feasible region of the internal light source exists the error. Therefore, feasible regions are not usually used for reconstructing during FMT reconstruction. Moreover, it is very difficult to use the feasible region effectively when the internal light source of the imaging region is in a deep position or there are multiple fluorescent light sources [81]. In order to improve these problems, researchers have proposed posterior feasible regions and optimized feasible regions [82, 83], and have been verified by numerical simulation models. The advantage of optimizing the feasible region is that it takes the whole imaging space as the initial feasible region, and optimizes the feasible region by iteratively updating the feasible region by judging the region where the light source is most likely to appear. On this basis, the researchers also proposed a feasible method of regional adaptive correction to further improve the applicability of feasible regions [84, 85].
The optical parameters (photon absorption coefficient and scattering coefficient) of each node in imaging space are the key factors that affect the reconstruction effect of FMT, and are the sufficient conditions that affect the photon propagation model and the accuracy of the reconstruction method [86,87,88,89]. The classical FMT method assumes imaging space as homogeneous space, that is, the optical parameters of each point in imaging space are the same. This assumption greatly simplifies the reconstruction operation, computational efficiency [90]. However, in biological conditions, the imaging space is usually not homogeneous, the optical parameters of various organs and tissues are far apart, and the reflection effects of different optical transmission media exist. Therefore, the researchers combined the prior information of the structure into the FMT reconstruction, proposed a nonhomogeneous imaging space model and a priori reconstruction method, which greatly improved the reconstruction accuracy. The structure of imaging space prior information can usually be obtained by highresolution structural imaging modalities such as computed tomography (CT), magnetic resonance imaging (MRI) [91,92,93,94,95,96]. The optical parameters of various organs and tissues can be obtained by other imaging techniques such as diffuse optical tomography (DOT) [97]. The imaging technique that combines imaging modalities to increase imaging prior information is also known as MultiModality imaging and is the focus of current medical imaging research [4].
Although researchers have put forward priori knowledge such as feasible regions, structural prior information to augment the information needed for reconstruction, the morbidity of the FMT reconstruction equation remains unresolved. Moreover, the actual FMT acquisition data usually contains a certain amount of noise, which has a great impact on the reconstruction of the pathological eq. A small signal disturbance may lead to a large reconstruction error. Therefore, researchers apply regularization techniques to FMT reconstruction to constrain the reconstruction process and reduce morbidity [8, 9, 28, 30, 63, 98,99,100,101,102,103,104,105,106,107,108,109,110,111,112,113,114,115]. The main principle of regularization is as follows:
where Ψ is the data fitting function, v is the regularization term, and λ is the regularization parameter, which is used to balance two items in Eq. (7). In FMT reconstruction, Ψ is usually chosen least squares, i.e. \( {\left\Vert AXb\right\Vert}_2^2 \). Recently, researchers proposed a new data fit term that yielded better reconstructions [116]. The regularization term choice of eq. (7) is the key to reducing morbidity and suppressing noise. Lpnorm regularization is the commonly used regularization method for FMT reconstruction, which is \( {\left\Vert X\right\Vert}_p^p \) [100, 114, 117]. The classical regularization term is L_{2} regularization, that is p = 2. The L_{2} norm regularization usually obtains a smoother reconstructed result of a large reconstructed area and has a good reconstruction effect for a large light source volume in an imaging space. Moreover, L_{2} norm can be derived mathematically, so it can be solved by many classical optimization methods, which is mathematically complete [118]. However, L_{2} norm reconstruction artifacts are usually large, and the phenomenon of smoothing occurs during the reconstruction, which is not conducive to the reconstruction of the complex structure of the light source.
Based on the regularization method, researchers began to try to apply the prior knowledge of light source to the regularization method. The priori knowledge that is currently used in regularization methods is the sparsity of fluorescent light sources. It is based on the assumption that the space occupied by a fluorescent light source is relatively sparse with respect to the whole imaging space or the distribution in the imaging space is sparse. In the actual biological tumor model, when the tumor is in the early stage of development, the tumor volume is relatively small; the distribution is more dispersed, more in line with the above assumptions. The regularization method based on sparsity is developed from the theory of compressed sensing (CS). Its basic principle is based on the signal sparsity characteristics, which recover the original signal from the missing collected information. L_{1} norm regularization (p = 1) is the mainstream sparse reconstruction method applied to FMT reconstruction today, which can reconstruct a good fluorescence threedimensional distribution image based on less fluorescence acquisition information [10, 98, 108, 109, 113]. Numerical simulation, physical simulation and in vivo experiments verify that regularization of L_{1} norm and L_{2} norm regularization can achieve more accurate reconstruction results [113]. However, L_{1} norm regularization works well in reconstructing a sparse light source, but overconvergence also exists. Moreover, regularization of the L_{1} norm also makes it difficult to reconstruct good images when the fluorescent light source does not meet the sparsity characteristics. The comparison of the L_{1} norm and L_{2} norm regularization is shown in Fig. 2.
Besides the L_{1} norm and L_{2} norm regularization, other forms of Lp norm regularization were utilized in the reconstruction of FMT [100, 114, 117, 119, 120]. These regularization methods (0 ≤ p ≤ 1) not only make full use of the gradient information of the objective functions, like the Tikhonov method, but also retain the advantages of the sparsity regularizations in improving image quality.
Another available regularization method is total variation (TV) [102, 121]. TV norm regularization was first proposed by Rudin et al. and has been applied to image denoising, DOT, photoacoustic imaging and BLT in recent years [65, 122,123,124,125]. The main idea of TV norm regularization is to constrain the variation terms of the distribution of the fluorescent light sources while preserving the boundaries of the light source zones. However, the TV norm regularization implies the following assumptions: the region of the fluorescent light source and the surrounding nonfluorescent light source region have the characteristics of piecewise constant, that is, the light intensity difference between the light source region and the nonlight source region is relatively large, and in each region light intensity is relatively constant [126,127,128]. In FMT reconstruction, FMT imaging satisfies the above assumptions when the property of the imaging internal light source is relatively simple (e.g., just one tumor). Therefore, the TV norm can be better applied to FMT reconstruction. The disadvantage of TV norm is its nonsmoothness and nondifferentiable, which makes it difficult to calculate. The traditional optimization method is difficult to apply TV norm solution. In order to solve this problem, the researchers put forward a variety of targeted optimization methods. SplitBregman method is one of the representatives [129]. It decomposes the coupled complex optimization problems in TV norm regularization into two relatively independent suboptimization problems, which can effectively improve the reconstruction effect. The experimental results show that the TV norm can get better reconstruction results. However, further analysis and improvement are needed. The comparison of L_{1} norm and TV norm regularization is shown in Fig. 3.
The reconstruction effect of regularization method is usually related to the choice of regularization parameter λ. In general, when the regularization parameter is small, many artefacts are generated due to noise amplification. When the regularization parameter is larger, the reconstructed image will have smoothed or over converged. To this end, researchers have proposed a series of methods for selecting appropriate regularization parameters such as Lcurve, Ucurve, crossvalidation, discrepancy principle [130, 131]. However, Vogel pointed out that the choice of regularization parameters should be associated with a specific inverse problem. The above methods have been widely used in DOT and FMT reconstruction [132, 133].
Based on the regularization model of FMT, there is a large number of solving methods to solve the inverse problem, such as Bayesianbased method [95, 112, 134], iterated shrinkage [29, 72, 118], pursuit method [135, 136], Newtonbased gradient descent method such as conjugate gradient method [109, 110, 137,138,139], SplitBregman method [10, 129, 136, 140], etc. These optimization methods achieved good result in FMT reconstruction especially in the balance of solving accuracy and computation efficiency. However, in general, the accuracy, efficiency and robustness cannot get the optimization at the same time. Take Newtonbased method as an example, it could achieve relatively robustness and accurate results of FMT, but the computation efficiency is unsatisfactory. Recently, in addition to proposing the new solving methods, lots of work concentrate on the improvement of these traditional optimization methods to improve the reconstruction performance, such as the utilization of structure priori [10, 141, 142], reweighted method [9, 108, 118], etc.
Conclusion
In this paper, we proposed the recent methodology advances in FMT. We briefly introduced the photon propagation model for FMT based on the radiative transfer equation, and further elaborated the solution method of forward problem and inverse problem based on the photon propagation model. We summarized the current research progress in the methodology of FMT, and focused on improving the accuracy, speed, and robustness of FMT.
As an important part of molecular imaging technology, the research and application of FMT has made rapid progress in the past decades because of the wide variety of probes and strong imaging signals. The outstanding advantages of low experimental cost and noninvasive in vivo observation have been widely used in many preclinical and clinical studies in recent years. However, there still remain difficulties to be solved for FMT which are as follows:

1.
More accurate and efficient photon propagation theory should be proposed. With the rapid development of computer hardware and software, especially with the support of largescale parallel processing technology and its corresponding largescale computer workstations, complex models (such as RTE) that could not be solved before are likely to become a reality today, and studies are more efficient. Higherorder calculus imaging model solution method is also one of the future research priorities.

2.
Recently, studies on in vivo imaging are mainly based on the use of specific probes to target in vivo tumors. In this regard, the excitation FMT technique in these researches is still in a preliminary stage and has not been widely implemented. Different from the implanted light source and simulation model, it is difficult for noninvasive reallife tumors to obtain information on the lesion inside the organism. Invasive imaging methods, such as frozen section imaging, have a certain degree of deformation in the same body condition. Therefore, how to verify the accuracy of reconstruction is also a problem that needs further study.

3.
The goal of FMT is to obtain structural distribution information at the cellular and molecular level targeted to the lesion area, strongly dependent on photon propagation in biological tissues, and the physiological and pathological information that can be acquired by FMT has limitations. Integrating optics, structure, and functional imaging with each other, and multiangle, systematic and comprehensive acquisition of multimodal fusion imaging has become the current research trend in the field of molecular imaging. At present, the more mature fusionexcited fluorescence tomography is combined with structural/optical multimode imaging represented by CT and MRI, functional/optical multimode imaging represented by PET, and optical/structure/functional multimodality image fusion. Both have received extensive attention and have published many highlevel results in preclinical and clinical applications and imaging theory. In the future work, the methodology in multimode fusion imaging need to be studied, to extend the existing imaging theory and methods to multimode fusion imaging, and further improve the application of FMT and imaging quality.
References
 1.
Blankenberg FG, Strauss HW. Recent advances in the molecular imaging of programmed cell death: part IInonprobebased MRI, ultrasound, and optical clinical imaging techniques. J Nucl Med. 2013;54:1–4.
 2.
Chi C, Du Y, Ye J, Kou D, Qiu J, Wang J. Intraoperative imagingguided cancer surgery: from current fluorescence molecular imaging methods to future multimodality imaging technology. Theranostics. 2014;4:1072–84.
 3.
Hu Z, Qu Y, Wang K, Zhang X, Zha J, Song T, Bao C, Liu H, Wang Z, Wang J, Liu Z, Liu H, Tian J. In vivo nanoparticlemediated radiopharmaceuticalexcited fluorescence molecular imaging. Nat Commun. 2015;6:7560.
 4.
Wang K, Chi C, Hu Z, Liu M, Hui H, Shang W, Peng D, Zhang S, Ye J, Liu H, Tian J. Optical molecular imaging Frontiers in oncology: the pursuit of accuracy and sensitivity. Engineering. 2015;1:309–23.
 5.
Chenouard N, Smal I, de Chaumont F, Maska M, Sbalzarini IF, Gong Y, et al. Objective comparison of particle tracking methods. Nat Methods. 2014;11:281–9.
 6.
Leng CC, Tian J. Mathematical method in optical molecular imaging. Sci China Inform Sci. 2015;58:1–13.
 7.
Qin CH, Feng JC, Zhu SP, Ma XB, Zhong JH, Wu P, Jin ZY, Tian J. Recent advances in bioluminescence tomography: methodology and system as well as application. Laser Photonics Rev. 2014;8:94–114.
 8.
Wang K, Wang Q, Luo Q, Yang X. Fluorescence molecular tomography in the second nearinfrared window. Opt Express. 2015;23:12669–79.
 9.
Xie W, Deng Y, Wang K, Yang X, Luo Q. Reweighted L1 regularization for restraining artifacts in FMT reconstruction images with limited measurements. Opt Lett. 2014;39:4148–51.
 10.
Zhang S, Wang K, Liu HB, Leng CC, Gao Y, Tian J. Reconstruction method for in vivo bioluminescence tomography based on the split Bregman iterative and surrogate functions. Mol Imaging Biol. 2017;19:245–55.
 11.
FanMinogue H, Cao Z, Paulmurugan R, Chan CT, Massoud TF, Felsher DW, Gambhir SS. Noninvasive molecular imaging of cMyc activation in living mice. Proc Natl Acad Sci U S A. 2010;107:15892–7.
 12.
Maji D, Solomon M, Nguyen A, Pierce RA, Woodard PK, Akers WJ, Achilefu S, Culver JP, Abendschein DR, Shokeen M. Noninvasive imaging of focal atherosclerotic lesions using fluorescence molecular tomography. J Biomed Opt. 2014;19(11):110501.
 13.
Miller JP, Maji D, Lam J, Tromberg BJ, Achilefu S. Noninvasive depth estimation using tissue optical properties and a dualwavelength fluorescent molecular probe in vivo. Biomed Opt Express. 2017;8:3095–109.
 14.
van Dam GM, Koller M, Qiu SQ, Linssen MD, de Vries J, Jansen L, Kelder W, de Jong JS, JorritsmaSmit A, van der Vegt B, Robinson DJ, Nagengast WB. Phase II inhuman dose escalation study of the optical molecular imaging tracer bevacizumab800cw for molecular fluorescence guided surgery in primary breast cancer patients. Cancer Res. 2017;7777:P40101P040101.
 15.
van Dam GM, Themelis G, Crane LM, Harlaar NJ, Pleijhuis RG, Kelder W, Sarantopoulos A, de Jong JS, Arts HJ, van der Zee AG, Bart J, Low PS, Ntziachristos V. Intraoperative tumorspecific fluorescence imaging in ovarian cancer by folate receptoralpha targeting: first inhuman results. Nat Med. 2011;17:1315–9.
 16.
Whitney MA, Crisp JL, Nguyen LT, Friedman B, Gross LA, Steinbach P, Tsien RY, Nguyen QT. Fluorescent peptides highlight peripheral nerves during surgery in mice. Nat Biotechnol. 2011;29:352–6.
 17.
Zhang Y, Yin G, Zhao H, Ma W, Gao F, Zhang L. Assessing pharmacokinetics of indocyanine greenloaded nanoparticle in tumor with a dynamic diffuse fluorescence tomography system. SPIE BiOS. 2018;10497:7.
 18.
Solomon SB, Cornelis F. Interventional molecular imaging. J Nucl Med. 2016;57:493–6.
 19.
Tarvainen T, Vauhkonen M, Kolehmainen V, Kaipio JP. Hybrid radiativetransferdiffusion model for optical tomography. Appl Opt. 2005;44:876–86.
 20.
Chen X, Gao X, Chen D, Ma X, Zhao X, Shen M, Li X, Qu X, Liang J, Ripoll J, Tian J. 3D reconstruction of light flux distribution on arbitrary surfaces from 2D multiphotographic images. Opt Express. 2010;18:19876–93.
 21.
Chandrasekhar S. Radiative transfer. New York: Dover Publications Inc; 1960.
 22.
Ren K, Abdoulaev GS, Bal G, Hielscher AH. Algorithm for solving the equation of radiative transfer in the frequency domain. Opt Lett. 2004;29:578–80.
 23.
Sreerekha TR, Buehler SA, Emde C. A simple new radiative transfer model for simulating the effect of cirrus clouds in the microwave spectral region. J Quant Spectrosc Ra. 2002;75:611–24.
 24.
Binzoni T, Leung TS, Gandjbakhche AH, Ruefenacht D, Delpy DT. The use of the HenyeyGreenstein phase function in Monte Carlo simulations in biomedical optics. Phys Med Biol. 2006;51:N313–22.
 25.
Pfeiffer N, Chapman GH. Successive order, multiple scattering of twoterm HenyeyGreenstein phase functions. Opt Express. 2008;16:13637–42.
 26.
Toublanc D. HenyeyGreenstein and Mie phase functions in Monte Carlo radiative transfer computations. Appl Opt. 1996;35:3270–4.
 27.
Tualle JM, Tinet E. Derivation of the radiative transfer equation for scattering media with a spatially varying refractive index. Opt Commun. 2003;228:33–8.
 28.
Guo X, Liu X, Wang X, Tian F, Liu F, Zhang B, Hu G, Bai J. A combined fluorescence and microcomputed tomography system for small animal imaging. IEEE Trans Biomed Eng. 2010;57:2876–83.
 29.
Han D, Yang X, Liu K, Qin C, Zhang B, Ma X, Tian J. Efficient reconstruction method for L1 regularization in fluorescence molecular tomography. Appl Opt. 2010;49:6930–7.
 30.
Hyde D, Miller EL, Brooks DH, Ntziachristos V. Data specific spatially varying regularization for multimodal fluorescence molecular tomography. IEEE Trans Med Imaging. 2010;29:365–74.
 31.
Lin Y, Bolisay L, Ghijsen M, Kwong TC, Gulsen G. Temperaturemodulated fluorescence tomography in a turbid media. Appl Phys Lett. 2012;100:73702–737024.
 32.
Lin Y, Kwong TC, Bolisay L, Gulsen G. Temperaturemodulated fluorescence tomography based on both concentration and lifetime contrast. J Biomed Opt. 2012;17:056007.
 33.
Song X, Wang D, Chen N, Bai J, Wang H. Reconstruction for freespace fluorescence tomography using a novel hybrid adaptive finite element algorithm. Opt Express. 2007;15:18300–17.
 34.
Zhang B, Yang X, Qin C, Liu D, Zhu S, Feng J, Sun L, Liu K, Han D, Ma X, Zhang X, Zhong J, Li X, Yang X, Tian J. A trust region method in adaptive finite element framework for bioluminescence tomography. Opt Express. 2010;18:6477–91.
 35.
Aydin ED, de Oliveira CR, Goddard AJ. A comparison between transport and diffusion calculations using a finite elementspherical harmonics radiation transport method. Med Phys. 2002;29:2013–23.
 36.
Grella K, Schwab C. Sparse tensor spherical harmonics approximation in radiative transfer. J Comput Phys. 2011;230:8452–73.
 37.
Guo HB, Hou YQ, He XW, Yu JJ, Cheng JX, Pu X. Adaptive hp finite element method for fluorescence molecular tomography with simplified spherical harmonics approximation. J Innov Opt Health Sci. 2014;7:1350057.
 38.
Han D, Tian J, Liu K, Feng J, Zhang B, Ma X, Qin C. Sparsitypromoting tomographic fluorescence imaging with simplified spherical harmonics approximation. IEEE Trans Biomed Eng. 2010;57:2564–7.
 39.
Khan T, Thomas A. Comparison of PN or spherical harmonics approximation for scattering media with spatially varying and spatially constant refractive indices. Opt Commun. 2005;255:130–66.
 40.
Klose AD. The forward and inverse problem in tissue optics based on the radiative transfer equation: a brief review. J Quant Spectrosc Radiat Transf. 2010;111:1852–3.
 41.
Klose AD, Larsen EW. Light transport in biological tissue based on the simplified spherical harmonics equations. J Comput Phys. 2006;220:441–70.
 42.
Duderstadt JJ, Martin WR. Transport Theory. New York: John Wiley; 1979.
 43.
Simon RA, Jeremy CH. Optical imaging in medicine: II. Modelling and reconstruction. Phys Med Biol. 1997;42:841.
 44.
Rasmussen JC, Joshi A, Pan T, Wareing T, McGhee J, SevickMuraca EM. Radiative transport in fluorescenceenhanced frequency domain photon migration. Med Phys. 2006;33:4685–700.
 45.
Arridge SR, Dehghani H, Schweiger M, Okada E. The finite element model for the propagation of light in scattering media: a direct method for domains with nonscattering regions. Med Phys. 2000;27:252–64.
 46.
Alexandrakis G, Farrell TJ, Patterson MS. Monte Carlo diffusion hybrid model for photon migration in a twolayer turbid medium in the frequency domain. Appl Opt. 2000;39:2235–44.
 47.
Hayashi T, Kashio Y, Okada E. Hybrid Monte Carlodiffusion method for light propagation in tissue with a lowscattering region. Appl Opt. 2003;42:2888–96.
 48.
Wang L, Jacques SL. Hybrid model of Monte Carlo simulation and diffusion theory for light reflectance by turbid media. J Opt Soc Am A Opt Image Sci Vis. 1993;10:1746–52.
 49.
Tarvainen T, Vauhkonen M, Kolehmainen V, Kaipio JP. Finite element model for the coupled radiative transfer equation and diffusion approximation. Int J Numer Meth Eng. 2006;65:383–405.
 50.
Martelli F, Sassaroli A, Yamada Y, Zaccanti G. Analytical approximate solutions of the timedomain diffusion equation in layered slabs. J Opt Soc Am A Opt Image Sci Vis. 2002;19:71–80.
 51.
Lian LC, Deng Y, Xie WH, Xu GQ, Yang XQ, Zhang ZH, et al. Enhancement of the localization and quantitative performance of fluorescence molecular tomography by using linear nBorn method. Opt Express. 2017;25:2063–79.
 52.
Wang X, Cao X, Zhang B, Liu F, Luo JW, Bai J. A hybrid reconstruction algorithm for fluorescence tomography using Kirchhoff approximation and finite element method. Med Biol Eng Comput. 2013;51:7–17.
 53.
Shen H, Wang G. A tetrahedronbased inhomogeneous Monte Carlo optical simulator. Phys Med Biol. 2010;55:947–62.
 54.
Alerstam E, Svensson T, AnderssonEngels S. Parallel computing with graphics processing units for highspeed Monte Carlo simulation of photon migration. J Biomed Opt. 2008;13:060504.
 55.
Quan G, Gong H, Deng Y, Fu J, Luo Q. Monte Carlobased fluorescence molecular tomography reconstruction method accelerated by a cluster of graphic processing units. J Biomed Opt. 2011;16:026018.
 56.
Ren N, Liang J, Qu X, Li J, Lu B, Tian J. GPUbased Monte Carlo simulation for light propagation in complex heterogeneous tissues. Opt Express. 2010;18:6811–23.
 57.
Cong AX, Hofmann MC, Cong W, Xu Y, Wang G. Monte Carlo fluorescence microtomography. J Biomed Opt. 2011;16:070501.
 58.
Cong W, Wang G. Boundary integral method for bioluminescence tomography. J Biomed Opt. 2006;11:020503.
 59.
Qin C, Tian J, Yang X, Liu K, Yan G, Feng J, et al. Galerkinbased meshless methods for photon transport in the biological tissue. Opt Express. 2008;16:20317–33.
 60.
Lu Y, Zhang X, Douraghy A, Stout D, Tian J, Chan TF, Chatziioannou AF. Source reconstruction for spectrallyresolved bioluminescence tomography with sparse a priori information. Opt Express. 2009;17:8062–80.
 61.
Wright S, Schweiger M, Arridge SR. Reconstruction in optical tomography using the PN approximations. Meas Sci Technol. 2007;18:79–86.
 62.
An Y, Liu J, Zhang G, Jiang S, Ye J, Chi C, et al. Compactly supported radial basis functionbased meshless method for photon propagation model of fluorescence molecular tomography. IEEE Trans Med Imaging. 2017;36:366–73.
 63.
Shi JW, Udayakumar TS, Wang ZQ, Dogan N, Pollack A, Yang YD. Optical molecular imagingguided radiation therapy part 2: integrated xray and fluorescence molecular tomography. Med Phys. 2017;44:4795–803.
 64.
Wang RX, Zhang DG, Zhu LF, Wen XO, Chen JX, Kuang CF, et al. Selectable surface and bulk fluorescence imaging with Plasmoncoupled waveguides. J Phys Chem C. 2015;119:22131–6.
 65.
Paulsen KD, Jiang H. Enhanced frequencydomain optical image reconstruction in tissues through totalvariation minimization. Appl Opt. 1996;35:3447–58.
 66.
Yu DF, Fessler JA. Edgepreserving tomographic reconstruction with nonlocal regularization. IEEE T Med Imaging. 1998;21:159–73.
 67.
Zhu W, Wang Y, Yao Y, Chang J, Graber HL, Barbour RL. Iterative total leastsquares image reconstruction algorithm for optical tomography by the conjugate gradient method. J Opt Soc Am A Opt Image Sci Vis. 1997;14:799–807.
 68.
Darne C, Lu Y, SevickMuraca EM. Small animal fluorescence and bioluminescence tomography: a review of approaches, algorithms and technology update. Phys Med Biol. 2014;59:R1–64.
 69.
Ale A, Ermolayev V, Herzog E, Cohrs C, de Angelis MH, Ntziachristos V. FMTXCT: in vivo animal studies with hybrid fluorescence molecular tomographyXray computed tomography. Nat Methods. 2012;9:615–20.
 70.
Berninger MT, Mohajerani P, Kimm M, Masius S, Ma X, Wildgruber M, et al. Fluorescence molecular tomography of DiRlabeled mesenchymal stem cell implants for osteochondral defect repair in rabbit knees. Eur Radiol. 2017;27:1105–13.
 71.
Deliolanis NC, Ale A, Morscher S, Burton NC, Schaefer K, Radrich K, et al. Deeptissue reportergene imaging with fluorescence and optoacoustic tomography: a performance overview. Mol Imaging Biol. 2014;16:652–60.
 72.
Han D, Tian J, Zhu S, Feng J, Qin C, Zhang B, Yang X. A fast reconstruction algorithm for fluorescence molecular tomography with sparsity regularization. Opt Express. 2010;18:8630–46.
 73.
Mohajerani P, Hipp A, Willner M, Marschner M, TrajkovicArsic M, Ma X, et al. FMTPCCT: hybrid fluorescence molecular tomographyxray phasecontrast CT imaging of mouse models. IEEE Trans Med Imaging. 2014;33:1434–46.
 74.
Mohajerani P, Ntziachristos V. An inversion scheme for hybrid fluorescence molecular tomography using a fuzzy inference system. IEEE Trans Med Imaging. 2016;35:381–90.
 75.
Ntziachristos V. Fluorescence molecular imaging. Annu Rev Biomed Eng. 2006;8:1–33.
 76.
Ntziachristos V. Going deeper than microscopy: the optical imaging frontier in biology. Nat Methods. 2010;7:603–14.
 77.
Yang FG, Ozturk MS, Yao RY, Intes X. Improving mesoscopic fluorescence molecular tomography through data reduction. Biomed Opt Express. 2017;8:3868–81.
 78.
Zacharakis G, Kambara H, Shih H, Ripoll J, Grimm J, Saeki Y, Weissleder R, Ntziachristos V. Volumetric tomography of fluorescent proteins through small animals in vivo. Proc Natl Acad Sci U S A. 2005;102:18252–7.
 79.
Cong W, Wang G, Kumar D, Liu Y, Jiang M, Wang L, Hoffman E, McLennan G, McCray P, Zabner J, Cong A. Practical reconstruction method for bioluminescence tomography. Opt Express. 2005;13:6756–71.
 80.
Wang G, Cong W, Durairaj K, Qian X, Shen H, Sinn P, Hoffman E, McLennan G, Henry M. In vivo mouse studies with bioluminescence tomography. Opt Express. 2006;14:7801–9.
 81.
Balima O, Charette A, Marceau D. Comparison of light transport models based on finite element and the discrete ordinates methods in view of optical tomography applications. J Comput Appl Math. 2010;234:2259–71.
 82.
Feng J, Jia K, Yan G, Zhu S, Qin C, Lv Y, et al. An optimal permissible source region strategy for multispectral bioluminescence tomography. Opt Express. 2008;16:15640–54.
 83.
Lv Y, Tian J, Cong W, Wang G, Yang W, Qin C, et al. Spectrally resolved bioluminescence tomography with adaptive finite element analysis: methodology and simulation. Phys Med Biol. 2007;52:4497–512.
 84.
Naser MA, Patterson MS. Algorithms for bioluminescence tomography incorporating anatomical information and reconstruction of tissue optical properties. Biomed Opt Express. 2010;1:512–26.
 85.
Naser MA, Patterson MS. Improved bioluminescence and fluorescence reconstruction algorithms using diffuse optical tomography, normalized data, and optimized selection of the permissible source region. Biomed Opt Express. 2010;2:169–84.
 86.
Alexandrakis G, Rannou FR, Chatziioannou AF. Tomographic bioluminescence imaging by use of a combined opticalPET (OPET) system: a computer simulation feasibility study. Phys Med Biol. 2005;50:4225–41.
 87.
Gibson AP, Hebden JC, Arridge SR. Recent advances in diffuse optical imaging. Phys Med Biol. 2005;50:R1–43.
 88.
Milstein AB, Stott JJ, Oh S, Boas DA, Millane RP, Bouman CA, et al. Fluorescence optical diffusion tomography using multiplefrequency data. J Opt Soc Am A. 2004;21:1035–49.
 89.
Ripoll J, Schulz RB, Ntziachristos V. Freespace propagation of diffuse light: theory and experiments. Phys Rev Lett. 2003;91:103901.
 90.
Ntziachristos V, Tung CH, Bremer C, Weissleder R. Fluorescence molecular tomography resolves protease activity in vivo. Nat Med. 2002;8:757–60.
 91.
Allard M, Cote D, Davidson L, Dazai J, Henkelman RM. Combined magnetic resonance and bioluminescence imaging of live mice. J Biomed Opt. 2007;12:034018.
 92.
Cao XH, Yang JH, Gao YZ, Guo YR, Wu GR, Shen DG. Dualcore steered nonrigid registration for multimodal images via bidirectional image synthesis. Med Image Anal. 2017;41:18–31.
 93.
Chen ZY, Wang YX, Yang F, Lin Y, Zhou QL, Liao YY. New researches and application progress of commonly used optical molecular imaging technology. Biomed Res Int. 2014;2014:429198.
 94.
Phillips EH, Di Achille P, Bersi MR, Humphrey JD, Goergen CJ. Multimodality imaging enables detailed hemodynamic simulations in dissecting aneurysms in mice. IEEE T Med Imaging. 2017;36:1297–305.
 95.
Xie WH, Deng Y, Yan DM, Yang XQ, Luo QM. Sparsitypromoting Bayesian approximation error method for compensating for the mismodeling of optical properties in fluorescence molecular tomography. Opt Lett. 2017;42:3024–7.
 96.
Zhang Y, Zhang B, Liu F, Luo J, Bai J. In vivo tomographic imaging with fluorescence and MRI using tumortargeted duallabeled nanoparticles. Int J Nanomedicine. 2014;9:33–41.
 97.
Wu LH, Zhao HJ, Wang X, Yi X, Chen WT, Gao F. Enhancement of fluorescence molecular tomography with structuralpriorbased diffuse optical tomography: combating optical background uncertainty. Appl Opt. 2014;53:6970–82.
 98.
An Y, Liu J, Zhang G, Ye J, Du Y, Mao Y, et al. A novel region reconstruction method for fluorescence molecular tomography. IEEE Trans Biomed Eng. 2015;62:1818–26.
 99.
Baikejiang R, Zhao Y, Fite BZ, Ferrara KW, Li CQ. Anatomical imageguided fluorescence molecular tomography reconstruction using kernel method. J Biomed Opt. 2017;22(5):55001.
 100.
Baritaux JC, Hassler K, Unser M. An efficient numerical method for general L(p) regularization in fluorescence molecular tomography. IEEE Trans Med Imaging. 2010;29:1075–87.
 101.
Cao X, Zhang B, Wang X, Liu F, Liu K, Luo J, et al. An adaptive Tikhonov regularization method for fluorescence molecular tomography. Med Biol Eng Comput. 2013;51:849–58.
 102.
Dutta J, Ahn S, Li C, Cherry SR, Leahy RM. Joint L1 and total variation regularization for fluorescence molecular tomography. Phys Med Biol. 2012;57:1459–76.
 103.
Guo H, Yu J, He X, Hou Y, Dong F, Zhang S. Improved sparse reconstruction for fluorescence molecular tomography with L1/2 regularization. Biomed Opt Express. 2015;6:1648–64.
 104.
He XL, Wang XD, Yi HJ, Chen YR, Zhang X, Yu JJ, et al. Laplacian manifold regularization method for fluorescence molecular tomography. J Biomed Opt. 2017;22(4):45009.
 105.
Lian L, Deng Y, Xie W, Xu G, Yang X, Zhang Z, et al. Highdynamicrange fluorescence molecular tomography for imaging of fluorescent targets with large concentration differences. Opt Express. 2016;24:19920–33.
 106.
Martin S, Simon RA, Ilkka N. Gauss–Newton method for image reconstruction in diffuse optical tomography. Phys Med Biol. 2005;50:2365.
 107.
Pera V, Brooks DH, Niedre M. Multiplexed fluorescence tomography with spectral and temporal data: demixing with intrinsic regularization. Biomed Opt Express. 2016;7:111–31.
 108.
Shi J, Liu F, Pu H, Zuo S, Luo J, Bai J. An adaptive support driven reweighted L1regularization algorithm for fluorescence molecular tomography. Biomed Opt Express. 2014;5:4039–52.
 109.
Shi J, Liu F, Zhang G, Luo J, Bai J. Enhanced spatial resolution in fluorescence molecular tomography using restarted L1regularized nonlinear conjugate gradient algorithm. J Biomed Opt. 2014;19:046018.
 110.
Shi J, Zhang B, Liu F, Luo J, Bai J. Efficient L1 regularizationbased reconstruction for fluorescent molecular tomography using restarted nonlinear conjugate gradient. Opt Lett. 2013;38:3696–9.
 111.
Yang F, Ozturk MS, Zhao L, Cong W, Wang G, Intes X. Highresolution mesoscopic fluorescence molecular tomography based on compressive sensing. IEEE Trans Biomed Eng. 2015;62:248–55.
 112.
Zhang GL, Liu F, Liu J, Luo JW, Xie YQ, Bai J, et al. Cone beam Xray luminescence computed tomography based on Bayesian method. IEEE T Med Imaging. 2017;36:225–35.
 113.
Zhang J, Shi J, Guang H, Zuo S, Liu F, Bai J, Luo J. Iterative correction scheme based on discrete cosine transform and L1 regularization for fluorescence molecular tomography with background fluorescence. IEEE Trans Biomed Eng. 2016;63:1107–15.
 114.
Zhao L, Yang H, Cong W, Wang G, Intes X. L(p) regularization for early gate fluorescence molecular tomography. Opt Lett. 2014;39:4156–9.
 115.
Zhou Y, Chen MM, Su H, Luo JW. Selfprior strategy for organ reconstruction in fluorescence molecular tomography. Biomed Opt Express. 2017;8:4671–86.
 116.
Gao H, Zhao H. Multilevel bioluminescence tomography based on radiative transfer equation part 1: l1 regularization. Opt Express. 2010;18:1854–71.
 117.
Wu P, Liu K, Zhang Q, Xue ZW, Li YB, Ning NA, et al. Detection of mouse liver cancer via a parallel iterative shrinkage method in hybrid optical/microcomputed tomography imaging. J Biomed Opt. 2012;17(12):126012.
 118.
Ping W, Yifang H, Kun W, Jie T. Bioluminescence tomography by an iterative reweighted (l)2 norm optimization. IEEE Trans Biomed Eng. 2014;61:189–96.
 119.
Jiang S, Liu J, An Y, Zhang G, Ye J, Mao Y, et al. Novel l 2,1norm optimization method for fluorescence molecular tomography reconstruction. Biomed Opt Express. 2016;7:2342–59.
 120.
Wang BY, Wan WB, Wang YH, Ma WJ, Zhang LM, Li J, et al. An Lp(0 <= p <= 1)norm regularized image reconstruction scheme for breast DOT with nonnegativeconstraint. Biomed Eng Online. 2017;16:32.
 121.
Freiberger M, Clason C, Scharfetter H. Total variation regularization for nonlinear fluorescence tomography with an augmented Lagrangian splitting approach. Appl Opt. 2010;49:3741–7.
 122.
Hyman JM, Flaschka H, Busse FH. Physica D. The ACM Digital Library: Elsevier Science Publishers B. V.; 1992.
 123.
Vese LA, Osher SJ. Image denoising and decomposition with total variation minimization and oscillatory functions. J Math Imaging Vis. 2004;20:7–18.
 124.
Yao L, Jiang H. Photoacoustic image reconstruction from fewdetector and limitedangle data. Biomed Opt Express. 2011;2:2649–54.
 125.
Yao L, Jiang HB. Enhancing finite elementbased photoacoustic tomography using total variation minimization. Appl Opt. 2011;50:5031–41.
 126.
Cerussi A, Hsiang D, Shah N, Mehta R, Durkin A, Butler J, et al. Predicting response to breast cancer neoadjuvant chemotherapy using diffuse optical spectroscopy. Proc Natl Acad Sci U S A. 2007;104:4014–9.
 127.
Ma X, Cheng Z, Jin Y, Liang X, Yang X, Dai Z, et al. SM51conjugated PLA nanoparticles loaded with 5fluorouracil for targeted hepatocellular carcinoma imaging and therapy. Biomaterials. 2014;35:2878–89.
 128.
Uddin MJ, Crews BC, Blobaum AL, Kingsley PJ, Gorden DL, McIntyre JO, et al. Selective visualization of cyclooxygenase2 in inflammation and cancer by targeted fluorescent imaging agents. Cancer Res. 2010;70:3618–27.
 129.
Feng J, Qin C, Jia K, Zhu S, Liu K, Han D, et al. Total variation regularization for bioluminescence tomography with the split Bregman method. Appl Opt. 2012;51:4501–12.
 130.
Hansen BPC. Analysis of discrete Illposed problem by means of LCurve. Soc Industr Appl Mathem Rev. 1992;34: 561–80.
 131.
Hansen PC, Nagy JG, O’Leary DP. Deblurring images : matrices, spectra, and filtering. J Electron Imaging. 2006;17:019901.
 132.
ChamorroServent J, Aguirre J, Ripoll J, Vaquero JJ, Desco M. Feasibility of Ucurve method to select the regularization parameter for fluorescence diffuse optical tomography in phantom and small animal studies. Opt Express. 2011;19:11490–506.
 133.
Chaudhari AJ, Ahn S, Levenson R, Badawi RD, Cherry SR, Leahy RM. Excitation spectroscopy in multispectral optical fluorescence tomography: methodology, feasibility and computer simulation studies. Phys Med Biol. 2009;54:4687–704.
 134.
Zhang G, Pu H, He W, Liu F, Luo J, Bai J. Bayesian framework based direct reconstruction of fluorescence parametric images. IEEE Trans Med Imaging. 2015;34:1378–91.
 135.
Ye J, Chi C, Xue Z, Wu P, An Y, Xu H, et al. Fast and robust reconstruction for fluorescence molecular tomography via a sparsity adaptive subspace pursuit method. Biomed Opt Express. 2014;5:387–406.
 136.
Ye JZ, Du Y, An Y, Mao YM, Jiang SX, Shang WT, et al. Sparse reconstruction of fluorescence molecular tomography using variable splitting and alternating direction scheme. Mol Imaging Biol. 2018;20:37–46.
 137.
Guo HB, He XW, Liu MH, Zhang ZY, Hu ZH, Tian J. Weight multispectral reconstruction strategy for enhanced reconstruction accuracy and stability with Cerenkov luminescence tomography. IEEE T Med Imaging. 2017;36:1337–46.
 138.
Petibon Y, Rakvongthai Y, El Fakhri G, Ouyang J. Direct parametric reconstruction in dynamic PET myocardial perfusion imaging: in vivo studies. Phys Med Biol. 2017;62:3539–65.
 139.
Rad JA, Parand K, Abbasbandy S. Local weak form meshless techniques based on the radial point interpolation (RPI) method and local boundary integral equation (LBIE) method to evaluate European and American options. Commun Nonlinear Sci. 2015;22:1178–200.
 140.
Hu Y, Liu J, Leng C, An Y, Zhang S, Wang K. L p regularization for bioluminescence tomography based on the split Bregman method. Mol Imaging Biol. 2016;18:830–7.
 141.
Liu Y, Liu J, An Y, Jiang S, Ye J, Mao Y, et al. Novel trace norm regularization method for fluorescence molecular tomography reconstruction. In: Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVI; 2017. p. 10047.
 142.
Teodori L, Crupi A, Costa A, Diaspro A, Melzer S, Tarnok A. Threedimensional imaging technologies: a priority for the advancement of tissue engineering and a challenge for the imaging community. J Biophotonics. 2017;10:24–45.
Acknowledgements
This paper is supported by Ministry of Science and Technology of China under Grant No. 2017YFA0205200, 2016YFC0103702, National Natural Science Foundation of China under Grant No. 81227901, 81527805, Chinese Academy of Sciences under Grant No. GJJSTD20170004, Beijing Municipal Science & Technology Commission No. Z161100002616022, and in part by the General Financial Grant from the China Postdoctoral Science Foundation under Grant 2017 M620952. The authors would like to acknowledge the instrumental and technical support of Multimodal biomedical imaging experimental platform, Institute of Automation, Chinese Academy of Sciences.
Author information
Affiliations
Contributions
YA carried out the major reference research in fluorescence molecular tomography studies, and drafted the manuscript. KW participated in the manuscript writing. JT conceived of the study and participated in its design and coordination. All authors read and approved the final manuscript.
Corresponding author
Correspondence to Jie Tian.
Ethics declarations
Competing interests
The authors declare that they have no competing interests.
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
About this article
Cite this article
An, Y., Wang, K. & Tian, J. Recent methodology advances in fluorescence molecular tomography. Vis. Comput. Ind. Biomed. Art 1, 1 (2018) doi:10.1186/s4249201800016
Received
Accepted
Published
DOI
Keywords
 Fluorescence molecular tomography
 Image reconstruction
 Photon propagation model
 Forward problem
 Inverse problem