- Original Article
- Open Access
Adaptive deep learning for head and neck cancer detection using hyperspectral imaging
Visual Computing for Industry, Biomedicine, and Art volume 2, Article number: 18 (2019)
It can be challenging to detect tumor margins during surgery for complete resection. The purpose of this work is to develop a novel learning method that learns the difference between the tumor and benign tissue adaptively for cancer detection on hyperspectral images in an animal model. Specifically, an auto-encoder network is trained based on the wavelength bands on hyperspectral images to extract the deep information to create a pixel-wise prediction of cancerous and benign pixel. According to the output hypothesis of each pixel, the misclassified pixels would be reclassified in the right prediction direction based on their adaptive weights. The auto-encoder network is again trained based on these updated pixels. The learner can adaptively improve the ability to identify the cancer and benign tissue by focusing on the misclassified pixels, and thus can improve the detection performance. The adaptive deep learning method highlighting the tumor region proved to be accurate in detecting the tumor boundary on hyperspectral images and achieved a sensitivity of 92.32% and a specificity of 91.31% in our animal experiments. This adaptive learning method on hyperspectral imaging has the potential to provide a noninvasive tool for tumor detection, especially, for the tumor whose margin is indistinct and irregular.
Orophary cancer is a common cancer worldwide and in recent years its incidence increased in a fast pace in both America and Europe . More than half a million patients receive the diagnosis of squamous-cell carcinoma of the head and neck worldwide each year . Survival rate of patients relates directly to the size of the primary tumor at first diagnosis, hence, early detection can be helpful in curing the disease completely. Squamous-cell carcinoma of the head and neck is a complex disease, which can be biopsied for histopathological assessment to make a definitive diagnosis traditionally. That is not only time consuming and invasive, but also subjective and inconsistent .
Hyperspectral imaging (HSI) is a technology that can acquire a series of images in many adjacent narrow spectral bands and reconstruct the reflectance spectrum for every pixel of the image . By measuring the reflection and absorption of the lights at different wavelengths, HSI has the ability to simultaneously provide information about different tissue constituents and their spatial distribution from the spectral signature of each pixel in the hyperspectral image . Hence, HSI technique can be applied in the noninvasive detection and diagnosis of cancer, such as breast cancer, gastric cancer, tongue cancer, and so on .
Hyperspectral images, known as hypercubes, contain rich information on a wide range of spectra with a high spectral resolution , hence, dimensionality reduction, image processing, and machine learning techniques are applied to extract the useful information from the vast amounts of HSI data, and have made many of the advancements in cancer identification: (1) Dimensionality reduction techniques. The principal component analysis [8, 9], tensor decompositions , and T-distributed stochastic neighbor approach [11, 12], were to reduce the dimensionality of features in hyperspectral images for compact expression; (2) Image processing techniques. Fourier coefficients , normalized difference nuclear index , sparse representation , box-plot and the watershed method , superpixel method , markov random fields [17, 18], and morphological method , were used for hyperspectral image processing and quantification analysis; (3) Machine learning techniques. Many of the advancements have been done in cancer identification using traditional machine learning classification models, such as linear discriminant analysis [20,21,22,23,24,25,26], quadratic discriminant analysis , support vector machine [12, 17, 20,21,22, 27,28,29,30,31,32,33,34,35,36,37], decision trees , k-nearest neighbors algorithm [22, 38], k-means [12, 19, 39], naïve bayes , random forests [21, 22, 34, 37], maximum likelihood , minimum spanning forest , gaussian mixture models , and semantic texton forest , and artificial neural network [33,34,35, 37], and so on.
However, these technologies require domain-specific knowledge to extract discriminant data to convert suitable features. In contrast to these conventional machine learning techniques, deep learning models can learn representations of data with multiple levels of abstraction, thereby can discover intricate structures in high-dimensional data with very little engineering by hand . Convolutional neural network (CNN) is a type of feed-forward artificial neural network, which has many successes in image recognition, natural language understanding, and medical image analysis . It also can improve the detection and classification performance on HSI . An HSI-based optical biopsy method was proposed using CNN, which could provide multi-category diagnostic information for normal head-and-neck tissue, squamous-cell carcinoma, and thyroid carcinomas [45,46,47]. A CNN-based modeling framework was introduced for the analysis of hyperspectral images for the detection of head and neck cancer in an animal model . A modified inception-v4 CNN architecture was used to detect the squamous cell carcinoma . In addition, several CNN-based architectures with pixel-wise prediction have shown their efficiency in the segmentation or detection task, such as fully connected networks (FCN) , SegNet , and U-Net . The U-Net deep neural network was used for the tumor segmentation  and the breast tumor detection  in hyperspectral images.
Since hyperspectral imagery has the system noise and image artifacts arising from uneven surface illumination, the tumor margin is irregular and unclear. So it is difficult to distinguish a tumor from surrounding normal tissue. In this study, we proposed an automated cancer detection algorithm for highlighting the tumor by adaptive auto-encoder network learning. Auto-encoder is an unsupervised deep neural network that can learn the inherent features and extract the suitable representation from complex data automatically. We involved the auto-encoder network to learn and recognize the depth features of pixels in hyperspectral imagery for the initial cancer detection. Each pixel is assigned a weight according to its classification result. The proposed adaptive auto-encoder learning method is performed on these weighted pixels and is trained to correct the misclassified pixels for the improvement of the detection performance. In this study, we demonstrate the efficiency and effectiveness of the auto-encoder and adaptive deep learning in HSI for head and neck cancer detection in an animal model. The method and experiments are described in the following sections.
Hyperspectral images were obtained by a wavelength-scanning CRI Maestro in vivo imaging system. This instrument mainly consists of a flexible fiber-optic lighting system, a solid-state liquid crystal filter, a spectrally optimized lens, and a 16-bit high-resolution charge-coupled device. For image acquisition, the wavelength setting can be defined within the range of 450 to 950 nm with 2-nm increments. Further details can be referred in our previous paper [10, 55].
The proposed adaptive deep learning method
The proposed adaptive deep learning method for cancer detection on HSI contains four parts: pre-processing, deep feature learning, adaptive weight learning, and post-processing. Figure 1 shows the overview of the method. After the input hypercube is preprocessed, deep feature is extracted and learned for the initial cancer detection. According to the output hypothesis of pixels, the adaptive weights are calculated and the updated hypercube is constructed. The discriminant deep feature is re-extracted and re-learned on the new hypercube. Hence, the re-trained model is adaptive and discriminative. Then, the cancerous tissue in a test hypercube can be identified by the adaptive model, and the detected cancerous tissue is refined by a post-processing step.
The acquired hyperspectral images were saved in a raw format, and correction was made with a white and a dark reference to remove the influence of the dark current and obtain a relative reflectance image. The corrected image, I, is calculated by
where Iraw is the raw image, Iwhite is the white reference image (100% reflectance) obtained by placing a standard white reference board in the field of view, and Idark is the dark image (0% reflectance) was acquired by keeping the camera shutter closed. These reference images were used to calibrate hyperspectral raw data before image analysis.
Deep feature learning
An auto-encoder is a type of artificial neural network used to learn efficient data coding in an unsupervised manner . It has one visible layer of k inputs, one hidden layer of d units, one reconstruction layer of k units, and an activation function. We illustrate the architectures of an auto-encoder in Fig. 2.
An auto-encoder consists of two parts, the encoder and the decoder. The encoder is to map the input X ∈ Rk to the hidden layer and produce the latent activity Y ∈ Rd. The decoder is to map Y to an output Z ∈ Rk, having the same number of nodes as the input layer, and with the purpose of reconstructing its own inputs X. We can get the Y and Z by
where w1 and w2 are the weight of input-to-hidden and the hidden-to-output, and b1 and b2 are their bias, f(p) is the activation function. In our method, it is a sigmoid function like:
Based on the structure of the auto-encoder, we use the spectral features of each pixel as the input, and train the network by iteratively updating the weights and biases to minimize the loss function of reconstruction error. To improve the robustness of the auto-encoder, in addition to the mean squared error between the input features and the reconstructed features, the L2 regularization and the Kullback-Leibler divergence based sparsity regularization is incorporated into the loss function. The learned feature that lies in the hidden layer is a learned deeper feature. The decoder part is been removed and the softmax layer is added into the network for the classification of cancer and normal tissue. The framework is shown in the Fig. 3.
The auto-encoder network can identify the cancer pixels and healthy pixels. Then we can obtain the initial detection result of cancer and the output hypothesis of pixels.
Adaptive weight learning
Because of the characteristics of medical images, the tumor margin is irregular and indistinct. The values of pixels in tumor are similar with those of pixels in healthy tissue. So, it is difficult to distinguish the tumor from its surrounding normal tissue for any learning method. Hence, we highlight the tumor by adjusting its weight adaptively in two aspects according to the misclassified pixels. The misclassified pixels can be divided into two types, the health pixels which are misclassified into cancer (false positives) and the cancer pixels which are misclassified into health (false negatives). The adaptive weight is assigned to each pixel according to its output hypothesis from the initial model, and it can be calculated by
where mask is the initial cancer detection, Pij is the i-th and j-th pixel in the mask and weight (Pij) is the weight of Pij. The weight_se(Pij) means to focus on the weights of the false negative pixels and the weight_sp(Pij) means to focus on the weights of the false positive pixels. The se (mask) is the sensitivity of the initial cancer detection and the sp (mask) is the specificity of the initial cancer detection. The Pri (Pij) is the prediction of the initial model for the pixel Pij, true (Pij) is the true label of the pixel Pij, 0 means the healthy tissue and 1 means the cancer tissue.
Eq. (4) is the definition of weight. We can see that the weight is adaptive because it is related to the local output hypothesis of each pixel and global detection performance. Then the adaptive weights can be used to inform the image, for instance, the misclassified pixels need to change their values in order to become distinguishable from its surrounding tissue. With the help of the weights, the original image can be updated as:
where hypercube_update is the updated hypercube and hypercube is the original hypercube, hypercube_update(Pij) means the value of Pij in the updated hypercube and it is the product of the value of Pij in the original one and the weight of Pij. The main aim of updating the original hypercube is to highlight the tumor most conducive to learning adaptively. Based on the updated hypercube, the deep features can be retrained, and an adaptive auto-encoder network model can be obtained.
Eqs. (4) and (5) show that the initial detected tumor is refined in two ways. If the detected tumor lost some relevant regions, then weight_se() could assign the weights to those false negative pixels and the updated image could focus on those pixels. So the new auto-encoder learner on the updated images tends to expand the relevant pixels to improve the sensitivity of tumor detection. If the detected tumor contained some irrelevant regions, then weight_sp() could assign the weights to those false positive pixels, and the updated image could highlight those pixels. So the new learner tends to eliminate the irrelevant pixels to improve the specificity of tumor detection. Thus, the pixels classified correctly keep their correct prediction while the pixels misclassified change their values adaptively. Therefore, the adaptive learner heightens the ability to identify the tumor and healthy tissue.
In this paper, the refined type improving the sensitivity or specificity will be determined by experiments according to the detection performance of training images.
Since our method is based on the classification of each pixel, the detected tumor may contain some noise and holes. The flood-fill operation is used to fill holes in the segmented binary image and the biggest connected component is chosen as our detected cancer tissue.
All methods were carried out in accordance with the approved Institutional Animal Care and Use Committee protocol (YER-2003103-042918BN) and the relevant guidelines and regulations of Emory University. We acquired the hyperspectral reflectance images from 12 tumor-bearing mice approximately 2 weeks post-tumor cell injection. The reflectance images contained 251 spectral bands and the image size on each spectral band was 390 × 435. Therefore, the data cube collected was a three-dimensional array of the size 390 × 435 × 251. In this study, tumor cells had green fluorescence protein (GFP) signals and thus GFP images were also acquired as the reference standard to evaluate the proposed tumor detection algorithm.
We conducted leave-one-out cross-validation experiments for the tumor detection in hyperspectral images. We take each hyperspectral image as the testing sample in turn, and the 11 remaining samples as the training samples.
The performance of auto-encoder could be affected by its reduced dimensionality. We test the dimension of compressed features from 20 to 60, step 5 on the training set. For each mouse, we train and test on its training set, and calculate the detection accuracy of each training samples, and obtain the average detection accuracy. The results of 12 mice named from #1 to #12 are shown in Fig. 4. The optimal dimensions of compressed feature are 55, 50, 40, 45, 60, 45, 55, 30, 60, 55, 60, and 60 corresponding to the highest accuracy for the 12 mice, respectively.
Next, we recorded the accuracy obtained by the adaptive sensitivity-weighted learning and specificity-weighted learning on the training set. The initial detected tumor of each mouse in the training set is refined by adjusting its sensitivity-weight and specificity-weight. The average accuracy is shown in Fig. 5. In Fig. 5, we can see that the adaptive sensitivity-weighted learning on the training sets for the mouse #1, #5, #6, #7, #10, and #11 has the higher accuracy than the adaptive specificity-weighted learning, while the adaptive specificity-weighted learning performs better on the other training sets. That shows the refine type we choose depends on the training data. Hence, for the mouse #1, #5, #6, #7, #10, and #11, we will choose the sensitivity-weighted learning for the improvement of cancer detection, and the mouse #2, #3, #4, #8, #9, and #12 will choose the specificity-weighted learning for the improvement.
Advantage of auto-encoder
To obtain the effective information, the auto-encoder is adopted to learn to compress the 251 wavelengths from 450 to 950 with 2 nm increments into a short feature by extracting the useful characters and ignoring noise. We randomly choose one mouse and use the auto-encoder to obtain the compressed features. Its average spectral values for the cancerous tissue and the healthy tissue of 251 wavelengths and 60 compassed features are shown in Fig. 6. The results in the Fig. 6 show that the difference of the spectral values between the cancerous tissue and the healthy tissue in the compassed features is bigger than that in the original wavelengths. The result demonstrates that auto-encoder can extract the deep features which descripting the discriminate characters for better distinguishing the cancer tissue from the health tissue.
In addition, we compare the auto-encoder with the CNN-based semantic segmentation neural network, such as FCN , SegNet , and U-Net . We train the FCN, SegNet, U-Net, and auto-encoder for the initial cancer detection on the HSI, and show the compared results in Fig. 7. In Fig. 7, we can see that the auto-encoder can perform best, so we choose the auto-encoder as our learner, and further improve its detection ability by using the adaptive learning.
Advantage of adaptive weight learning
To see the advantage of adaptive weight in the detection of cancer tissue, we compare the detection performance of the original learning method and the adaptive weight learning method. The compared results are shown in Fig. 8. Figure 8a is the original gray image by averaging the intensities of 251 spectral bands. It is difficult to identify the part edge of the cancer tissue. The model involved the auto-encoder and neural network learns on the image with 251 spectral bands, and predicts and shows the probability of cancer in Fig. 8b. In Fig. 8b, we can see that most pixels have been classified correctly, that proves the effectiveness of auto-encoder again. However, there are still some misclassified pixels. According to the classification results, the weight of each pixel has been calculated and the weight image is shown in Fig. 8c. In the weight image, we can see that a weight is assigned to each pixel. Based on the weight image, we obtain the new image as shown as Fig. 8d. The new image is tweaked in favor of those misclassified pixels. A new model trained on the new image can achieve better classification performance than that on the original image. The updated prediction result is shown in Fig. 8e. Compared to the gold standard of cancer detection (Fig. 8f), the updated cancer detection performance involved the adaptive weights is better than the original performance, that demonstrates that the adaptive weighted learning is beneficial to improve the accuracy of cancer detection.
We show the qualitative results of cancer detection on five mice in Fig. 9, where the RGB composite images generated from the tumor hypercube are shown in the first row, the initial cancer detection results by the auto-encoder learning are shown in the second row (the white parts mean the cancer tissue while the black parts mean the healthy tissue), the improved detection results by adaptive weighted auto-encoder learning are shown in the third row, the final results refined by post-processing are shown in the fourth row, and the gold standards are shown in the fifth row.
In Fig. 9, we can see that the initial cancer detection results are sensitive to the blood vessel and uneven surface. Since the improved cancer detection method focuses the misclassified pixels with the help of adaptive weight, it is robust to blood vessel, uneven surface and noise, like the detection results of the first and third mouse. Even it can achieve good performance when the tumor has the irregular or unclear margin, like the detection results of the second and fourth mouse. The final detection results refined by post-processing are satisfactory. However, we also can see the performance as shown in the last column is not satisfied. That because the difference between the tumor and normal tissue is too obscure, the intra-similarity between the tumor in the center and the tumor at the edge is much bigger than the inter-similarity between the tumor at the edge and the sounding normal tissue. But we can see that our adaptive auto-encoder method still works better than the auto-encoder method even though when an edge part of the tumor disappears. Hence, our adaptive weight learning is effective.
Table 1 provides the quantitative evaluation results of our proposed method for 12 mice. The average sensitivity, specificity, and accuracy of 12 mice are 92.32%, 91.31%, and 91.33%, respectively. These results confirm that our method is effective. Based on the above results, we know that the bad performances of the last two mice shown in Table 1 were caused by the tissue artifacts and the invasion of surrounding tissue. Although our method received unsatisfied results when part of tumors disappear, it could obtain above 80% accuracy. If we remove the two mice, the average sensitivity, specificity, and accuracy, will be 94.72%, 92.26%, and 92.97%, respectively.
In this study, we proposed automated detection method for head and neck cancer using the adaptive deep learning on hyperspectral imagery in an animal model. Auto-encoder network model is involved to extract the deep features from a hyperspectral imagery with size of 390 × 435 × 251 and distinguish the cancerous tissue from its surrounding normal tissue. Because of the noise and uneven surface and so on, the detected cancer region is not satisfactory. To improve the initial performance and obtain a complete tumor, the adaptive auto-encoder network model is proposed, which focus on the misclassified pixels and enhance to learn for the misclassified pixels. The method is shown to classify the tumor region with high sensitivity, specificity, and accuracy.
Since reflectance hyperspectral images contain 251 spectral bands from 450 to 950 nm with 2 nm increments, and each hyperspectral image contains over millions of reflectance spectral signatures, it is difficult to extract discriminant features from the huge data by hand. Deep learning methods can learn features by building high-level features from low-level ones and automatically discover the features needed for cancer detection. The auto-encoder is an unsupervised deep neural network that tries to denoise the inputs automatically by finding the latent representation from which to reconstruct the original input, hence it is especially suitable for describing the hyperspectral data. As shown in the Fig. 6, the extracted features by auto-encoder method can better distinguish the cancerous tissue from the non-cancerous tissues. In addition, the auto-encoder can achieve higher accuracy compared with the other neural network models, hence, the auto-encoder is used as the learner for the initial cancer detection.
Although auto-encoder can extract the useful information for detecting the cancer tissue, the blood vessel or uneven surface make it difficult to identify the complete tumor from the normal tissue. Since blood vessel or uneven surface could misrepresent the tissue structures, neither the traditional classification methods nor the deep learning methods could detect the tumor with high accuracy by learning the intensity or the distribution of intensity. The proposed adaptive auto-encoder network can predict the misrepresented tissue structures into their true classes by adaptively weighting those misclassified pixels, and thus greatly improve the performance of tumor detection, as shown in Figs. 7, 8, 9 and Table 1. Under the same conditions, our proposed method performed better than the traditional classification methods [10, 31], and the CNN based deep learning method [45, 50,51,52]. However, we obtain unsatisfied performance on some mice. In the next work, we plan to improve the cancer detection performance on those special images. We will learn the deep feature by iteratively updating the sensitivity-weights and specificity-weights until convergence to overcome the effects of noise and artifacts.
The automatic detection algorithm was written and run in MATLAB on Intel Core 2.60GHz CPU with 16GB of RAM. The time for normalization, deep feature extraction, cancer detection, post-processing is about 0.1 s, 2.8 s, 3.2 s, and 0.02 s, respectively. The total running time is about 6 s for per hyperspectral image. It greatly improved the efficiency of cancer detection compared with the method  using 45 min. This automatic cancer detection method can be implemented in real time if involving the multi-thread, GPU acceleration or parallel programming.
In this study, an adaptive deep learning framework was proposed and validated for head and neck cancer detection using HSI in an animal model. This algorithm extracted the deep feature of hyperspectral images for reducing the dimensionality effectively and better characterizing the cancerous tissue. The adaptive weight learning could improve the cancer detection performance by focusing on harder-to-classify pixels. In the head and neck cancer mouse model, the proposed cancer detection method was able to obtain a high sensitivity and specificity. The results demonstrated that the HSI combined with deep learning technique may enable accurate and fast detection of cancers in a noninvasive manner and may provide a promising tool for future clinical applications.
Availability of data and materials
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Convolutional neural network
Fully connected networks
Green fluorescence protein
Mehanna H, Beech T, Nicholson T, El-Hariry I, McConkey C, Paleri V et al (2013) Prevalence of human papillomavirus in oropharyngeal and nonoropharyngeal head and neck cancer-systematic review and meta-analysis of trends by time and region. Head Neck 35(5):747–755 https://doi.org/10.1002/hed.22015
Haddad RI, Shin DM (2008) Recent advances in head and neck cancer. N Engl J Med 359(11):1143–1154 https://doi.org/10.1056/NEJMra0707975
van den Brekel MWM, Lodder WL, Stel HV, Bloemena E, Leemans CR, van der Waal I (2012) Observer variation in the histopathologic assessment of extranodal tumor spread in lymph node metastases in the neck. Head Neck 34(6):840–845 https://doi.org/10.1002/hed.21823
Goetz AFH, Vane G, Solomon JE, Rock BN (1985) Imaging spectrometry for earth remote sensing. Science 228(4704):1147–1153 https://doi.org/10.1126/science.228.4704.1147
Calin MA, Parasca SV, Savastru D, Manea D (2013) Hyperspectral imaging in the medical field: present and future. Appl Spectrosc Rev 49(6):435–447 https://doi.org/10.1080/05704928.2013.838678
Lu GL, Fei BW (2014) Medical hyperspectral imaging: a review. J Biomed Opt 19(1):010901 https://doi.org/10.1117/1.JBO.19.1.010901
Borengasser M, Hungate WS, Watkins R (2007) Hyperspectral remote sensing: principles and applications. CRC Press, Boca Raton, FL, USA. DOI: https://doi.org/10.1201/9781420012606
Lazcano R, Madroñal D, Salvador R, Desnos K, Pelcat M, Guerra R et al (2017) Porting a PCA-based hyperspectral image dimensionality reduction algorithm for brain cancer detection on a manycore architecture. J Syst Arch 77:101–111 https://doi.org/10.1016/j.sysarc.2017.05.001
Chung H, Lu GL, Tian ZQ, Wang DS, Chen ZG, Fei BW (2016) Superpixel-based spectral classification for the detection of head and neck cancer with hyperspectral imaging. In: abstracts of SPIE 9788, medical imaging 2016: biomedical applications in molecular, structural, and functional imaging, SPIE, San Diego, CA, USA, 29 March 2016, p 978813 DOI: https://doi.org/10.1117/12.2216559
Lu GL, Halig LV, Wang DS, Qin XL, Chen ZG, Fei BW (2014) Spectral-spatial classification for noninvasive cancer detection using hyperspectral imaging. J Biomed Opt 19(10):106004 https://doi.org/10.1117/1.JBO.19.10.106004
Ravì D, Fabelo H, Callic GM, Yang GZ (2017) Manifold embedding and semantic segmentation for intraoperative guidance with hyperspectral brain imaging. IEEE Trans Med Imaging 36(9):1845–1857 https://doi.org/10.1109/TMI.2017.2695523
Fabelo H, Ortega S, Ravi D, Kiran BR, Sosa C, Bulters D et al (2018) Spatio-spectral classification of hyperspectral images for brain cancer detection during surgical operations. PLoS One 13(3):e0193721 https://doi.org/10.1371/journal.pone.0193721
Lu GL, Wang DS, Qin XL, Halig L, Muller S, Zhang HZ et al (2015) Framework for hyperspectral image processing and quantification for cancer detection during animal tumor surgery. J Biomed Opt 20(12):126012 https://doi.org/10.1117/1.JBO.20.12.126012
Siddiqi AM, Li H, Faruque F, Williams W, Lai K, Hughson M et al (2008) Use of hyperspectral imaging to distinguish normal, precancerous, and cancerous cells. Cancer 114(1):13–21 https://doi.org/10.1002/cncr.23286
Liu Z, Wang HJ, Li QL (2012) Tongue tumor detection in medical hyperspectral images. Sensors 12(1):162–174 https://doi.org/10.3390/s120100162
Regeling B, Laffers W, Gerstner AOH, Westermann S, Müller NA, Schmidt K et al (2016) Development of an image pre-processor for operational hyperspectral laryngeal cancer detection. J Biophotonics 9(3):235–245 https://doi.org/10.1002/jbio.201500151
Kho E, de Boer LL, Van de Vijver KK, Sterenborg HJCM, Ruers TJ (2018) Hyperspectral imaging for detection of breast cancer in resection margins using spectral-spatial classification. In: abstracts of SPIE 10472, diagnosis and treatment of diseases in the breast and reproductive system IV, SPIE, San Francisco, CA, USA, 14 March 2018, p 104720F DOI: https://doi.org/10.1117/12.2288367
Gopi A, Reshmi CS, Aneesh RP (2017) An effective segmentation algorithm for the hyperspectral cancer images. In: abstracts of 2017 international conference on networks & advances in computational technologies, IEEE, Thiruvanthapuram, India, 20 July 2017, pp 294-299 DOI: https://doi.org/10.1109/NETACT.2017.8076783
Zarei N, Bakhtiari A, Gallagher P, Keys M, MacAulay C (2017) Automated prostate glandular and nuclei detection using hyperspectral imaging. In: abstracts of the IEEE 14th international symposium on biomedical imaging, IEEE, Melbourne, VIC, Australia, 18 April 2017, pp 1028-1031 DOI: https://doi.org/10.1109/ISBI.2017.7950691
Fei BW, Lu GL, Halicek MT, Wang X, Zhang HZ, Little JV, et al (2017) Label-free hyperspectral imaging and quantification methods for surgical margin assessment of tissue specimens of cancer patients. In: abstracts of the 39th annual international conference of the IEEE engineering in medicine and biology society, IEEE, Seogwipo, South Korea, 11 July 2017, pp 4041-4045 DOI: https://doi.org/10.1109/EMBC.2017.8037743
Lu GL, Little JV, Wang X, Zhang HZ, Patel MR, Griffith CC et al (2017) Detection of head and neck cancer in surgical specimens using quantitative hyperspectral imaging. Clin Cancer Res 23(18):5426–5436 https://doi.org/10.1158/1078-0432.CCR-17-0906
Lu GL, Qin XL, Wang DS, Muller S, Zhang HZ, Chen A, et al (2016) Hyperspectral imaging of neoplastic progression in a mouse model of oral carcinogenesis. In: abstracts of SPIE 9788, medical imaging 2016: biomedical applications in molecular, structural, and functional imaging, SPIE, San Diego, CA, USA, 29 March 2016, p 978812 DOI: https://doi.org/10.1117/12.2216553
Fei BW, Lu GL, Wang X, Zhang HZ, Little JV, Patel MR et al (2017) Label-free reflectance hyperspectral imaging for tumor margin assessment: a pilot study on surgical specimens of cancer patients. J Biomed Opt 22(8):086009 https://doi.org/10.1117/1.JBO.22.8.086009
Beaulieu RJ, Goldstein SD, Singh J, Safar B, Banerjee A, Ahuja N (2018) Automated diagnosis of colon cancer using hyperspectral sensing. Int J Med Robot Comput Assist Surg 14(3):e1897 https://doi.org/10.1002/rcs.1897
de Koning SG, Karakullukcu MB, Smit L, Baltussen EJM, Sterenborg HJCM, Ruers TJM (2018) Near infrared hyperspectral imaging to evaluate tongue tumor resection margins intraoperatively. In: abstracts of SPIE 10469, optical imaging, therapeutics, and advanced technology in head and neck surgery and otolaryngology 2018, SPIE, San Francisco, CA, USA, 14 March 2018, p 104690G
Yuan X, Zhang D, Wang C, Dai B, Zhao M, Li B (2018) Hyperspectral imaging and SPA-LDA quantitative analysis for detection of colon cancer tissue. J Appl Spectrosc 85(2):307–312 https://doi.org/10.1007/s10812-018-0649-x
Akbari H, Halig L, Schuster DM, Fei BW, Osunkoya A, Master V et al (2012) Hyperspectral imaging and quantitative analysis for prostate cancer detection. J Biomed Opt 17(7):076005 https://doi.org/10.1117/1.JBO.17.7.076005
Lu GL, Wang DS, Qin XL, Muller SS, Wang X, Chen AY et al (2018) Detection and delineation of squamous neoplasia with hyperspectral imaging in a mouse model of tongue carcinogenesis. J Biophotonics 11(3):e201700078 https://doi.org/10.1002/jbio.201700078
Akbari H, Uto K, Kosugi Y, Kojima K, Tanaka N (2011) Cancer detection using infrared hyperspectral imaging. Cancer Sci 102(4):852–857 https://doi.org/10.1111/j.1349-7006.2011.01849.x
Akbari H, Halig LV, Zhang HZ, Wang DS, Chen ZG, Fei BW (2012) Detection of cancer metastasis using a novel macroscopic hyperspectral method. In: abstracts of SPIE 8317, medical imaging 2012: biomedical applications in molecular, structural, and functional imaging, SPIE, San Diego, CA, USA, 14 April 2012, p 831711 DOI: https://doi.org/10.1117/12.912026
Pike R, Lu GL, Wang DS, Chen ZG, Fei BW (2016) A minimum spanning forest-based method for noninvasive cancer detection with hyperspectral imaging. IEEE Trans Biomed Eng 63(3):653–663 https://doi.org/10.1109/TBME.2015.2468578
Torti E, Fontanella A, Florimbi G, Leporati F, Fabelo H, Ortega S et al (2018) Acceleration of brain cancer detection algorithms during surgery procedures using GPUs. Microprocess Microsyst 61:171–178 https://doi.org/10.1016/j.micpro.2018.06.005
Nathan M, Kabatznik AS, Mahmood A (2018) Hyperspectral imaging for cancer detection and classification. In: abstracts of the 3rd biennial south African biomedical engineering conference, IEEE, Stellenbosch, South Africa, 4 April 2018, pp 1-4 DOI: https://doi.org/10.1109/SAIBMEC.2018.8363180
Ortega S, Fabelo H, Camacho R, Plaza ML, Callico GM, Lazcano R et al (2017) P03.18 detection of human brain cancer in pathological slides using hyperspectral images. Neuro-Oncol 19(Suppl 3):iii37 https://doi.org/10.1093/neuonc/nox036.133
Ortega S, Callicó GM, Plaza ML, Camacho R, Fabelo H, Sarmiento R (2016) Hyperspectral database of pathological in-vitro human brain samples to detect carcinogenic tissues. In: abstracts of the IEEE 13th international symposium on biomedical imaging, IEEE, Prague, Czech Republic, 13 April 2016, pp 369-372 DOI: https://doi.org/10.1109/ISBI.2016.7493285
Calin MA, Parasca Sr SV, Manea D (2018) Comparison of spectral angle mapper and support vector machine classification methods for mapping skin burn using hyperspectral imaging. In: abstracts of SPIE 10677, unconventional optical imaging, SPIE, Strasbourg, France, 13 August 2018, p 106773P DOI: https://doi.org/10.1117/12.2319267
Ortega S, Fabelo H, Camacho R, De la Luz PM, Callicó GM, Sarmiento R (2018) Detecting brain tumor in pathological slides using hyperspectral imaging. Biomed Opt Express 9(2):818–831 https://doi.org/10.1364/BOE.9.000818
Florimbi G, Fabelo H, Torti E, Lazcano R, Madroñal D, Ortega S et al (2018) Accelerating the K-nearest neighbors filtering algorithm to optimize the real-time classification of human brain tumor in hyperspectral images. Sensors 18(7):2314 https://doi.org/10.3390/s18072314
Khouj Y, Dawson JM, Coad J, Vona-Davis L (2018) Hyperspectral imaging and K-means classification for histologic evaluation of ductal carcinoma in situ. Front Oncol 8:17 https://doi.org/10.3389/fonc.2018.00017
Lall M, Deal J, Hill S, Rider P, Boudreaux C, Rich T, Leavesley S (2017) Classification of normal and Lesional colon tissue using fluorescence excitation-scanning hyperspectral imaging as a method for early diagnosis of colon cancer. In: abstracts of the national conference on undergraduate research, University of Memphis, Memphis, TN, USA, 6-8 April 2017, pp 1063-1073
Regeling B, Thies B, Gerstner AOH, Westermann S, Müller NA, Bendix J et al (2016) Hyperspectral imaging using flexible endoscopy for laryngeal cancer detection. Sensors 16(8):1288 https://doi.org/10.3390/s16081288
LeCun Y, Bengio Y, Hinton G (2015) Deep learning. Nature 521(7553):436–444 https://doi.org/10.1038/nature14539
Schmidhuber J (2015) Deep learning in neural networks: an overview. Neural Netw 61:85–117 https://doi.org/10.1016/j.neunet.2014.09.003
Makantasis K, Karantzalos K, Doulamis A, Doulamis N. (2015) Deep supervised learning for hyperspectral data classification through convolutional neural networks. In: Abstracts of 2015 IEEE international geoscience and remote sensing symposium, IEEE, Milan, Italy, 26 July 2015, pp 4959–4962 DOI: https://doi.org/10.1109/IGARSS.2015.7326945
Halicek M, Little JV, Wang X, Chen AY, Fei BW (2019) Optical biopsy of head and neck cancer using hyperspectral imaging and convolutional neural networks. J Biomed Opt 24(3):036007 https://doi.org/10.1117/1.JBO.24.3.036007
Halicek M, Lu GL, Little JV, Wang X, Patel M, Griffith CC et al (2017) Deep convolutional neural networks for classifying head and neck cancer using hyperspectral imaging. J Biomed Opt 22(6):060503 https://doi.org/10.1117/1.JBO.22.6.060503
Halicek M, Little JV, Wang X, Patel M, Griffith CC, Chen AY, et al (2018) Tumor margin classification of head and neck cancer using hyperspectral imaging and convolutional neural networks. In: abstracts of SPIE 10576, medical imaging 2018: image-guided procedures, robotic interventions, and modeling, SPIE, Houston, TX, United States, 12 March 2018, p 1057605 DOI: https://doi.org/10.1117/12.2293167
Ma L, Lu GL, Wang DS, Wang X, Chen ZG, Muller S, et al (2017) Deep learning based classification for head and neck cancer detection with hyperspectral imaging in an animal model. In: abstracts of SPIE 10137, medical imaging 2017: biomedical applications in molecular, structural, and functional imaging, SPIE, Orlando, FL, USA, 13 March 2017, p 101372G DOI: https://doi.org/10.1117/12.2255562
Halicek M, Dormer JD, Little JV, Chen AY, Myers L, Sumer BD et al (2019) Hyperspectral imaging of head and neck squamous cell carcinoma for cancer margin detection in surgical specimens from 102 patients using deep learning. Cancers 11(9):1367 https://doi.org/10.3390/cancers11091367
Long J, Shelhamer E, Darrell T (2015) Fully convolutional networks for semantic segmentation. In: abstracts of 2015 IEEE conference on computer vision and pattern recognition, IEEE, Boston, MA, USA, 7-12 June 2015, pp 3431-3440 DOI: https://doi.org/10.1109/CVPR.2015.7298965
Badrinarayanan V, Kendall A, Cipolla R (2017) Segnet: a deep convolutional encoder-decoder architecture for image segmentation. IEEE Trans Pattern Anal Mach Intell 39(12):2481–2495 https://doi.org/10.1109/TPAMI.2016.2644615
Ronneberger O, Fischer P, Brox T (2015) U-net: convolutional networks for biomedical image segmentation. In: abstracts of the 18th international conference on medical image computing and computer-assisted intervention, Springer, Munich, Germany, 5-9 October 2015, pp 234-241 DOI: https://doi.org/10.1007/978-3-319-24574-4_28
Trajanovski S, Shan CF, Weijtmans PJC, de Koning, SGB, Ruers TJM (2019) Tumor semantic segmentation in hyperspectral images using deep learning. In: Abstracts Proceedings of the 2nd international conference on medical imaging with deep learning, MIDL, London, UK, 7 July 2019, pp 8–10
Kho E, Dashtbozorg B, de Boer LL, Van de Vijver KK, Sterenborg HJCM, Ruers TJM (2019) Broadband hyperspectral imaging for breast tumor detection using spectral and spatial information. Biomed Opt Express 10(9):4496–4515 https://doi.org/10.1364/BOE.10.004496
Lu GL, Halig L, Wang DS, Chen ZG, Fei BW (2014) Spectral-spatial classification using tensor modeling for cancer detection with hyperspectral imaging. In: abstracts of SPIE 9034, medical imaging 2014: image processing, SPIE, San Diego, CA, United States. 21 March 2014, p 903413 DOI: https://doi.org/10.1117/12.2043796
Hinton GE, Zemel RS (1993) Autoencoders, minimum description length and Helmholtz free energy. In: abstracts of the 6th international conference on neural information processing systems, Morgan Kaufmann publishers Inc., Denver, CO, USA, 2 December 1993, pp 3-10
The author thanks the members of the Quantitative Bioimaging Laboratory (https://www.fei-lab.org) for their helpful discussions.
All methods were carried out in accordance with the approved Institutional Animal Care and Use Committee (IACUC) protocol (YER-2003103-042918BN) and the relevant guidelines and regulations of Emory University.
This work was supported in part by NIH grants (R01CA204254, R01HL140325, and R21CA231911).
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Ma, L., Lu, G., Wang, D. et al. Adaptive deep learning for head and neck cancer detection using hyperspectral imaging. Vis. Comput. Ind. Biomed. Art 2, 18 (2019). https://doi.org/10.1186/s42492-019-0023-8
- Hyperspectral imaging
- Deep learning
- Adaptive learning
- Noninvasive cancer detection